Etin Besin İçeriğinin Belirlenme Yöntemleri
Özet
Et ve et ürünleri; yüksek kaliteli proteinler, esansiyel amino asitler, lipidler, B12 gibi önemli vitaminler ve mineraller bakımından zengin yapılarıyla insan beslenmesinde kritik öneme sahip son derece değerli gıda kaynaklarıdır. Hayvanın cinsi, beslenme şekli, kesim öncesi stres, yaşlandırma süresi ve depolama koşulları gibi çeşitli faktörlerden etkilenen et bileşiminin doğru bir şekilde analiz edilmesi; kalite kontrolünün sağlanması, ürün tutarlılığı ve gıda etiketleme standartlarına uyum açısından büyük önem taşımaktadır. Metinde, etin besinsel bileşenlerinin ve kalite parametrelerinin belirlenmesi amacıyla kullanılan temel analitik yöntemler ayrıntılı olarak ele alınmaktadır. Etteki nem ve kurumadde oranının belirlenmesinde etüvde kurutma, düşük basınç, mikrodalga, Karl-Fischer titrasyonu ve tahribatsız hızlı bir yöntem olan yakın kızılötesi spektroskopisi (NIRS) kullanılmaktadır. Toplam lipid ve yağ asidi içerikleri Goldfish, Soxhlet, Fosslet, Mojonnier, Folch ve Babcock metotları ile gaz kromatografisi (GC-FID) kullanılarak saptanırken, lipid oksidasyonu düzeyleri TBARS analiziyle ölçülmektedir. Protein miktarının hesaplanmasında toplam nitrojen tayinine dayanan Kjeldahl ve Dumas yöntemleri uygulanmaktadır. Mineral içerik kuru ve ıslak külleme teknikleriyle analiz edilirken, antioksidan kapasite ise DPPH, TEAC, ORAC ve süperoksit radikal söndürme metotlarıyla değerlendirilmektedir. Sonuç olarak, gelişen modern analitik teknikler et sanayisi ve tüketiciler için gıda güvenilirliği ile kalite standartlarının sürdürülmesinde hayati rol oynamaktadır.
Meat and meat products are highly valuable food sources of critical importance in human nutrition, owing to their rich composition of high-quality proteins, essential amino acids, lipids, key vitamins such as B12, and minerals. Accurate analysis of meat composition—which is influenced by various factors such as animal breed, diet, pre-slaughter stress, aging time, and storage conditions—holds great significance for ensuring quality control, product consistency, and compliance with food labeling standards. The text details the fundamental analytical methods used to determine the nutritional components and quality parameters of meat. Oven drying, low pressure, microwave, Karl-Fischer titration, and near-infrared spectroscopy (NIRS)—a rapid, non-destructive technique—are employed to determine moisture and dry matter content. Total lipid content and fatty acid profiles are determined using Goldfish, Soxhlet, Fosslet, Mojonnier, Folch, and Babcock methods alongside gas chromatography (GC-FID), while lipid oxidation levels are measured via TBARS analysis. For protein content calculation, the Kjeldahl and Dumas methods based on nitrogen determination are applied. Mineral content is analyzed using dry and wet ashing techniques, whereas antioxidant capacity is evaluated through DPPH, TEAC, ORAC, and superoxide radical scavenging assays. Consequently, advancing modern analytical techniques play a vital role in maintaining food safety and quality standards for both the meat industry and consumers.
Referanslar
Ahmad, M. M., Salim-Ur-Rehman, Iqbal, Z., Anjum, F. M., & Sultan, J. I. (2006). Genetic variability to essential oil composition in four citrus fruit species. Pakistan Journal of Botany, 38(2), 319–324.
Ahmad, S., Ahmad, R. S., & Hussain, M. B. (2018). Nutritional Composition of Meat. In Muhammad Sajid Arshad (Ed.), Meat Science and Nutrition (pp. 61–77). https://doi.org/10.5772/intechopen.77045
Al-Mamun, M., Yamaki, K., Masumizu, T., Nakai, Y., Saito, K., Sano, H., & Tamura, Y. (2007). Superoxide anion radical scavenging activities of herbs and pastures in northern Japan determined using electron spin resonance spectrometry. International Journal of Biological Sciences, 3(6), 349–355. https://doi.org/10.7150/ijbs.3.349
Alarcon-Rojo, A. D., Carrillo-Lopez, L. M., Reyes-Villagrana, R., Huerta-Jiménez, M., & Garcia-Galicia, I. A. (2019). Ultrasound and meat quality: A review. Ultrasonics Sonochemistry, 55(September 2018), 369–382. https://doi.org/10.1016/j.ultsonch.2018.09.016
Albayrak, S., Sağdıç, O., & Aksoy, A. (2010). Bitkisel ürünlerin ve gıdaların antioksidan kapasitelerinin belirlenmesinde kullanılan yöntemler. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 26(4), 401–409.
Alvisi, P., Brusa, S., Alboresi, S., Amarri, S., Bottau, P., & Cavagni, G. (2015). OF PEDIATRICS Recommendations on complementary feeding for healthy , full-term infants. ???, April. https://doi.org/10.1186/s13052-015-0143-5
Bradley, R. L. (2010). Moisture and Total Solids Analysis. In S. S. Nielsen (Ed.), Food Analyis (4th ed., pp. 87–104). Springer.
Büyüktuncel, E. (2012). Gelişmiş Ekstraksiyon Teknikleri I. Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, 32(2), 209–242.
Caldiroli, A., Maria, A., Capuzzi, E., Clerici, M., & Buoli, M. (2020). Journal of A ff ective Disorders Malondialdehyde and bipolar disorder : A short comprehensive review of available literature. Journal of Affective Disorders, 274(April), 31–37. https://doi.org/10.1016/j.jad.2020.05.001
Chapman, J., Elbourne, A., Truong, V. K., & Cozzolino, D. (2020). Review Shining light into meat – a review on the recent advances in in vivo and carcass applications of near infrared spectroscopy. 935–941. https://doi.org/10.1111/ijfs.14367
Clinquart, A., Ellies-Oury, M. P., Hocquette, J. F., Guillier, L., Santé-Lhoutellier, V., & Prache, S. (2022). Review: On-farm and processing factors affecting bovine carcass and meat quality. Animal, xxxx, 100426. https://doi.org/10.1016/j.animal.2021.100426
Dave, D., & Ghaly, A. (2011). Meat Spoilage Mechanisms and Preservation Techniques: A Critical Review. American Journal of Agricultural and Biological Sciences, 6, 486–510. https://doi.org/http://dx.doi.org/10.3844/ajabssp.2011.486.510
Devatkal, S. K., Thorat, P., & Manjunatha, M. (2012). Effect of vacuum packaging and pomegranate peel extract on quality aspects of ground goat meat and nuggets. Journal of Food Science and Technology, 51(10), 1–6. https://doi.org/10.1007/s13197-012-0753-5
Eggers, L. F., & Schwudke, D. (2020). Liquid Extraction:Folch. In Encyclopedia of Lipidomics (Vol. 3, pp. 1–6). https://doi.org/10.1007/978-94-007-7864-1
Harris, G. K., & Marshall, M. R. (2010). Ash Analysis. In S. S. Nielsen (Ed.), Food Analyis (Fourth Edi, pp. 107–114). Springer.
Higgs, J. D. (2000). The changing nature of red meat: 20 years of improving nutritional quality. Trends in Food Science and Technology, 11(3), 85–95. https://doi.org/10.1016/S0924-2244(00)00055-8
Hoffman, L. C., Kroucamp, M., & Manley, M. (2007). Meat quality characteristics of springbok (Antidorcas marsupialis). 2: Chemical composition of springbok meat as influenced by age, gender and production region. Meat Science, 76(4), 762–767.
Holman, B. W. B., Bailes, K. L., Meyer, R. G., & Hopkins, D. L. (2019). Effect of modified Soxhlet (Soxtec) and Folch extraction method selection on the total lipid determination of aged beef. Journal of Food Science and Technology, 56(8), 3957–3961. https://doi.org/10.1007/s13197-019-03878-4
Ismail, B. P. (2017). Ash Content Determination. In S. S. Nielsen (Ed.), Food Analyis Laboratory Manual (3rd ed., pp. 117–119). Springer.
Jamal, S. D., Jamil, D. M., Khidhir, Z. K., & Mark, C. (2020). Journal of Food and Dairy Sciences Protein Determination in some Animal Products from Sulaymaniyah Markets Using Kjeldahl Procedure. 11(12), 343–346. https://doi.org/10.21608/jfds.2020.160394
Jiang, B. (2014). Food Safety : Food Analysis Technologies / Techniques. 3. https://doi.org/10.1016/B978-0-444-52512-3.00052-8
Jiang, B., Tsao, R., Li, Y., & Miao, M. (2014). Food Safety : Food Analysis Technologies / Techniques. In Neal K. Van Alfen (Ed.), Encyclopedia of Agriculture and Food Systems (1st ed., Vol. 3, pp. 273–286). Elsevier Academic Press. https://doi.org/10.1016/B978-0-444-52512-3.00052-8
Jiang, X., Wu, S., Zhou, Z., & Akoh, C. C. (2015). Physicochemical properties and volatile profiles of cold-pressed Trichosanthes kirilowii maxim seed oils. International Journal of Food Properties, 19(8), 1765–1775. https://doi.org/10.1080/10942912.2015.1107731
Joshi, Y. B., & Praticò, D. (2014). Lipid Peroxidation in Psychiatric Illness : Overview of Clinical Evidence. 2014. https://doi.org/10.1155/2014/828702
Kademi, H. I., Ulusoy, B. H., & Hecer, C. (2019). Applications of miniaturized and portable near infrared spectroscopy ( NIRS ) for inspection and control of meat and meat products. Food Reviews International, 35(3), 201–220. https://doi.org/10.1080/87559129.2018.1514624
Kamruzzaman, M., Elmasry, G., Sun, D., & Allen, P. (2012). Analytica Chimica Acta Prediction of some quality attributes of lamb meat using near-infrared hyperspectral imaging and multivariate analysis. Analytica Chimica Acta, 714, 57–67. https://doi.org/10.1016/j.aca.2011.11.037
Karakök Göncü, S., Ozogul, Y., Saler, M., & Ozogul, F. (2008). Proximate analysis fatty acid profiles and mineral contents of meat: A comparative study. 21(2010), 210–223.
Kerry, Joseph, P., & Ledward, D. (2009). Improving the sensory and nutritional quality of fresh meat. Woodhead Publishing.
Kulkarni, V. V., Girish, P. S., Barbuddhe, S. B., Naveena, B. M., & Muthukumar, M. (2022). Moisure Estimation in Meat. In Analytical Techniques in Meat Science (1st ed., pp. 5–6). CRC Press.
Kumar, Y., Yadav, D. N., Ahmad, T., & Narsaiah, K. (2015). Recent Trends in the Use of Natural Antioxidants for Meat and Meat Products. Comprehensive Reviews in Food Science and Food Safety, 14, 796–812. https://doi.org/10.1111/1541-4337.12156
Liu, T., Zhong, S., Liao, X., Chen, J., He, T., & Lai, S. (2015). A Meta-Analysis of Oxidative Stress Markers in Depression. PLos ONE, 10(10), 1–17. https://doi.org/10.1371/journal.pone.0138904
Lucarini, M., Durazzo, A., Sánchez del Pulgar, J., Gabrielli, P., & Lombardi-Boccia, G. (2018). Determination of fatty acid content in meat and meat products: The FTIR-ATR approach. Food Chemistry, 267(March 2017), 223–230. https://doi.org/10.1016/j.foodchem.2017.11.042
Marini, D. (2006). Lipid Analyses in Food. In Encyclopedia of Analytical Chemistry (pp. 1–32). John Wiley & Sons, Ltd. https://doi.org/10.1002/9780470027318.a1015
Marshall, M. R. (2010). Ash Analysis. In S. S. Nielsen (Ed.), Food Analyis (Fourth, pp. 105–115).
Melro, E., Antunes, F., Cruz, I., Ramos, P. E., Carvalho, F., & Alves, L. (2020). Morphological, textural and physico-chemical characterization of processed meat products during their shelf life. Food Structure, 26(January). https://doi.org/10.1016/j.foostr.2020.100164
Mihaljev, Ž. A., Jakšić, S. M., Prica, N. B., Ćupić, Ž. N., & Baloš, M. M. Ž. (2015). Comparison of the Kjeldahl method , Dumas method and NIR method for total nitrogen determination in meat and meat products. Journal of Agroalimentary Processes and Technologies, 21(4), 365–370.
Min, D. B., & Ellefson, W. C. (2010). Fat Analysis. In S. S. Nielsen (Ed.), Food Analyis (pp. 117–132). Springer. https://doi.org/10.1007/978-1-4419-1478-1_8
Mirzadeh, M., Arianejad, M. R., & Khedmat, L. (2020). Antioxidant, antiradical, and antimicrobial activities of polysaccharides obtained by microwave-assisted extraction method: A review. Carbohydrate Polymers, 229(October 2019), 115421. https://doi.org/10.1016/j.carbpol.2019.115421
Nielsen, S. S. (2006). Proximate Assays in Food Analysis. In R. A. Meyers (Ed.), Encyclopedia of Analytical Chemistry: Applications, Theory and Instrumentation (pp. 1–8). https://doi.org/10.1002/9780470027318
Nielsen, S. S., & Carpenter, E. C. (2017). Fat Content Determination. In S. S. Nielsen (Ed.), Food Analyis Laboratory Manual (Third, pp. 121–127).
O`Keefe, S. F., & Pike, O. A. (2010). Fat Characterization. In S. S. Nielsen (Ed.), Food Analyis (40th ed., pp. 239–260).
Okan, O. T., Varlıbaş, H., Öz, M., & Deniz, İ. (2013). Antioksidan Analiz Yöntemleri ve Doğu Karadeniz Bölgesinde Antioksidan Kaynağı Olarak Kullanılabilecek Odun Dışı Bazı Bitkisel Ürünler. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 13(1), 48–59.
Osborne, B. G. (2006). Near-infrared Spectroscopy in Food Analysis. In R. A. Meyers (Ed.), Encyclopedia of Analytical Chemistry (pp. 1–14). https://doi.org/10.1002/9780470027318
Pérez-Palacios, T., Ruiz, J., Martín, D., Muriel, E., & Antequera, T. (2008). Comparison of different methods for total lipid quantification in meat and meat products. Food Chemistry, 110(4), 1025–1029. https://doi.org/10.1016/j.foodchem.2008.03.026
Perez, D., & Andujar, G. (1981). Various procedures for determining ’ ash " content of meat products have been compared . The ISO method gave significantly higher results than the other methods tested . The standard method ( IAS 2-043 ) gave lower recoveries and , in one of the experimen. 5(1978), 165–170.
Pettinati, J. D., Swift, C. E., & Cohen, E. H. (1973). Moisture and Fat Analysis of Meat and Meat Products: A Review and Comparison of Methods. Journal of AOAC INTERNATIONAL, 56(3), 544–561. https://doi.org/10.1093/jaoac/56.3.544
Pikul, J., Leszczynski, D. E., & Kummerow, F. A. (1989). Evaluation of Three Modified TBA Methods for Measuring Lipid Oxidation in Chicken Meat. 1309–1313.
Prieto, N., Roehe, R., Lavín, P., Batten, G., & Andrés, S. (2009). Application of near infrared reflectance spectroscopy to predict meat and meat products quality: A review. Meat Science, 83(2), 175–186. https://doi.org/10.1016/j.meatsci.2009.04.016
Richards, A. T., & Analyst, P. (2018). Food and Nutritional Analysis — Meat and Meat Products ☆. In Encyclopedia of Analytical Science, 3rd Edition (3rd ed.). Elsevier Inc. https://doi.org/10.1016/B978-0-12-409547-2.14511-1
Sacchetti, G., Mattia, C. Di, Pittia, P., & Martino, G. (2008). Application of a radical scavenging activity test to measure the total antioxidant activity of poultry meat. Meat Science, 80, 1081–1085. https://doi.org/10.1016/j.meatsci.2008.04.030
Seljeskog, E., Hervig, T., & Azam, M. (2006). A novel HPLC method for the measurement of thiobarbituric acid reactive substances ( TBARS ). A comparison with a commercially available kit. Clinical Biochemistry, 39, 947–954. https://doi.org/10.1016/j.clinbiochem.2006.03.012
Shah, M. A., John, S., Bosco, D., & Mir, S. A. (2014). Plant extracts as natural antioxidants in meat and meat products. MESC, 98(1), 21–33. https://doi.org/10.1016/j.meatsci.2014.03.020
Silva, L. C. R., Folli, G. S., Santos, L. P., Barros, I. H. A. S., Oliveira, B. G., Borghi, T., Francine, D., Filgueiras, P. R., & Rom, W. (2020). Quantification of beef , pork , and chicken in ground meat using a portable NIR spectrometer. Vibrational Spectroscopy, 111(103158). https://doi.org/10.1016/j.vibspec.2020.103158
Şimşek, A., & Birol, K. (2012). Et ve Et Ürünlerinde Antioksidan Kullanımındaki Güncel Geli ş meler. Akademik Gıda, 10(2), 75–83.
Soren, N. M., & Biswas, K. A. (2020). Methods for nutritional quality analysis of meat. In K. A. Biswas & K. P. Mandal (Eds.), Meat Quality Analysis (pp. 21–34). Academic Press, Elsevier.
Sørland, G. H., Larsen, P. M., Lundby, F., Rudi, A. P., & Guiheneuf, T. (2004). Determination of total fat and moisture content in meat using low field NMR. Meat Science, 66(3), 543–550. https://doi.org/10.1016/S0309-1740(03)00157-8
Su, H., Sha, K., Zhang, L., Zhang, Q., Xu, Y., Zhang, R., Li, H., & Sun, B. (2014). Development of near infrared reflectance spectroscopy to predict chemical composition with a wide range of variability in beef. Meat Science, 98(2), 110–114. https://doi.org/10.1016/j.meatsci.2013.12.019
Wang, J., Fan, L., Zhou, Q., Li, J., Zhao, P., Wang, Z., Zhang, H., Yan, S., & Huang, L. (2018). Rapid Determination of Meat Moisture Content Using Radio-Frequency Dielectric Measurement. IEEE Access, 6, 51384–51391. https://doi.org/10.1109/ACCESS.2018.2864308
Wang, X. (2019). Near-infrared spectroscopy for food quality evaluation. In J. Zhong & X. Wang (Eds.), Evaluation Technologies for Food Quality (pp. 105–118). https://doi.org/https://doi.org/10.1016/C2017-0-01187-4
Wood, J. D., Enser, M., Richardson, R. I., & Whittington, F. M. (2007). Fatty Acids in Meat and Meat Products. In C. Ching Kung (Ed.), Fatty Acids in Foods and their Health Implication (Third, pp. 87–106).
Yetim, H., & Kesmen, Z. (2009). Gıda Analizleri. Erciyes Üniversitesi Ders Kitapları.
