Biyoremediasyon ve Tarım
Özet
Sağlıklı beslenmenin temelini oluşturan tarım topraklarının tarımsal uygulamalarla kirletilmesi çağımızın acil çözüm bulunması gereken sorunlarından birini oluşturmaktadır. Gereğinden fazla gübre kullanımı, pestisitlerin yoğun şekilde uygulanması ve arıtılmamış sularla sulama gibi sebeplerle tarım topraklarında organik ve anorganik kirleticiler birikmekte, bu kirleticiler hem tarımsal üretimde verim düşüşüne sebep olmakta hem de besin zinciri yoluyla kirleticilerin hayvan ve insan sağlığına zararı dokunabilmektedir. Bu kirleticilerin temizlenmesinde geleneksel fiziksel ve kimyasal uygulamalar yerine ekolojik ve düşük maliyetli çözümler tercih edilmeye başlanmıştır. Bu yöntemlerden biri bakteri, alg ve mantarlar gibi mikroorganizmaların kullanıldığı biyoremediasyondur. Bu yöntemde mikroorganizmaların enzimleri ve metabolik faaliyetleri sayesinde kirletici bileşiklerinin kimyasal formlarında değişiklik meydana gelebilir, kirletici metabolik yolaklarda mikroorganizmaların karbon kaynağı olarak kullanılabilir veya mikroorganizmalar kirleticileri hücre içine alarak bitkilerin bünyesine geçişlerini engelleyebilir. Lipazlar, proteazlar, hidrolaz ve dehidrogenazlar gibi enzimler kirleticilerin gideriminde önemli rol oynarlar. Bu enzimleri yüksek düzeyde üretebilen mikroorganizmaların belirlenmesi, kirli toprakların bünyesindeki kirleticilerin ve konsantrasyonlarının ve söz konusu mikroorganizmaların varlığının belirlenmesi ve gerektiğinde bu mikroorganizmaların kirletilmiş topraklara eklenmesi başarılı bir biyoremediasyon sürecinin vazgeçilmezleridir. Biyoremediasyon sonucunda tarım toprakları yalnızca kirleticiden arındırılmış olmaz, aynı zamanda hem organik madde miktarı hem de toprak mikrobiyal florası desteklenmiş olur. Bu gibi sebeplerle çevre dostu, uygulaması kolay ve düşük maliyetli biyoremediasyon yöntemlerinin benimsenmesi son derece önemlidir.
The contamination of agricultural soils, which form the basis of healthy nutrition, through agricultural practices is one of the urgent problems of our time that requires a solution. Excessive fertilizer use, intensive pesticide application, and irrigation with untreated water cause organic and inorganic pollutants to accumulate in agricultural soils. These pollutants not only reduce agricultural productivity but also harm animal and human health through the food chain. Instead of traditional physical and chemical applications, ecological and ow-cost solutions are now being preferred. One of these methods is bioremediation where microorganisms such as bacteria, algae, and fungi are used. In this method, the enzymes and metabolic activities of microorganisms can cause changes in the chemical form of pollutants, pollutants can be used as a carbon source in metabolic pathways, or microorganisms can prevent the transfer of pollutants into plants by taking them into their cells. Enzymes such as lipases, proteases, hydrolases, and dehydrogenases play an important role in the removal of pollutants. Identifying microorganisms that can produce these enzymes at high levels, determining the pollutants and their concentrations in contaminated soils and the presence of these microorganisms, and adding these microorganisms to contaminated soils when necessary are essential for a successful bioremediation process. As a result of bioremediation, agricultural soils are not only decontaminated, but also have an increased organic matter content and the soil flora is enriched. For these reasons, it is extremely important to adopt environmentally friendly, easy-to-implement, and low-cost bioremediation methods.
Referanslar
Minami K. Soil is a living substance. Soil Science and Plant Nutrition. 2021;67(1): 26–30. https://doi.org/10.1080/00380768.2020.1827939.
Nuruzzaman M, Bahar MM, Naidu R. Diffuse soil pollution from agriculture: Impacts and remediation. Science of The Total Environment. 2025;962: 178398. https://doi.org/10.1016/J.SCITOTENV.2025.178398.
Gomiero T. Large-scale biofuels production: A possible threat to soil conservation and environmental services. Applied Soil Ecology. 2018;123: 729–736. https://doi.org/10.1016/J.APSOIL.2017.09.028.
Yeşilyurt S. Toprak Kirliliği. In: Horuz A (ed.) Toprak Bilgisi. Ankara: Akademisyen Yayınevi; 2025. p. 209237.
Rashid B, Husnain T, Riazuddin S. Herbicides and Pesticides as Potential Pollutants: A Global Problem. In: Ashraf M, Ozturk M, Ahmad MSA (eds.) Plant Adaptation and Phytoremediation. Springer, Dordrecht; 2010. p. 427–447. https://doi.org/10.1007/978-90-481-9370-7_19.
Miglioranza KSB, Ondarza PM, Grondona SI, Scenna LB. Persistent Organic Contaminants. In: Blasco J, Tovar-Sánchez A (eds.) Marine Analytical Chemistry. Springer, Cham; 2023. p. 275–306. https://doi.org/10.1007/978-3-031-14486-8_5.
Gürgan M, Yeşilyurt S. Fate and Behaviour of Heavy Metals in Soil Systems and Their Risk Assesment. In: Kazankaya A, Ateş MA (eds.) Current Approaches in Scientific Studies. Ankara: IKSAD Publishing House; 2024. https://doi.org/10.5281/zenodo.13136820.
Dudka S, Miller WP. Accumulation of potentially toxic elements in plants and their transfer to human food chain. Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes. 1999;34(4): 681–708. https://doi.org/10.1080/03601239909373221.
He L, Xu Y, Zhang M, Gul S, Zhang X, Zhong H, et al. Effect of remediation technologies on soil fertility in heavy metal(loid)-contaminated soils: A critical review. Critical Reviews in Environmental Science and Technology. 2024;54(19): 1417–1435. https://doi.org/10.1080/10643389.2024.2315009.
Rizzo L, Gernjak W, Krzeminski P, Malato S, McArdell CS, Perez JAS, et al. Best available technologies and treatment trains to address current challenges in urban wastewater reuse for irrigation of crops in EU countries. Science of The Total Environment. 2020;710: 136312. https://doi.org/10.1016/J.SCITOTENV.2019.136312.
Gürgan M, Yeşilyurt S. Bioremediation Of Toxic Level Plant Micro Nutrients In Wastewaters For Irrigation. In: Kazankaya A, Ateş MA (eds.) Advance Concepts On Natural And Agricultural Sciences. Ankara; 2023.
Ritchie H, Rosado P, Roser M. Data Page: Total pesticide use. Agricultural Production.
T.C. Tarım ve Orman Bakanlığı. Resmi Tarımsal İlaç İstatistikleri. 2024.
Defarge N, Spiroux de Vendômois J, Séralini GE. Toxicity of formulants and heavy metals in glyphosate-based herbicides and other pesticides. Toxicology Reports. 2018;5: 156–163. https://doi.org/10.1016/J.TOXREP.2017.12.025.
Azubuike CC, Chikere CB, Okpokwasili GC. Bioremediation techniques–classification based on site of application: principles, advantages, limitations and prospects. World Journal of Microbiology and Biotechnology 2016 32:11. 2016;32(11): 180-. https://doi.org/10.1007/S11274-016-2137-X.
Singh P, Singh VK, Singh R, Borthakur A, Madhav S, Ahamad A, et al. Bioremediation: a sustainable approach for management of environmental contaminants. Abatement of Environmental Pollutants: Trends and Strategies. 2020; 1–23. https://doi.org/10.1016/B978-0-12-818095-2.00001-1.
Silva VP, Moreira-Santos M, Mateus C, Teixeira T, Ribeiro R, Viegas CA. Evaluation of Arthrobacter aurescens Strain TC1 as Bioaugmentation Bacterium in Soils Contaminated with the Herbicidal Substance Terbuthylazine. PLOS ONE. 2015;10(12): e0144978. https://doi.org/10.1371/JOURNAL.PONE.0144978.
Tyagi M, da Fonseca MMR, de Carvalho CCCR. Bioaugmentation and biostimulation strategies to improve the effectiveness of bioremediation processes. Biodegradation 2010 22:2. 2010;22(2): 231–241. https://doi.org/10.1007/S10532-010-9394-4.
19.Kanissery RG, Sims GK. Biostimulation for the Enhanced Degradation of Herbicides in Soil. Applied and Environmental Soil Science. 2011;2011(1): 843450. https://doi.org/10.1155/2011/843450.
Harekrushna S, Chandan Kumar D. A Review on: Bioremediation. International Journal of Research in Chemistry and Environment. 2012;2: 13–21.
Juwarkar AA, Singh SK, Mudhoo A. A comprehensive overview of elements in bioremediation. Reviews in Environmental Science and Biotechnology. 2010. p. 215–288. https://doi.org/10.1007/s11157-010-9215-6.
Dindar E, Şağban Topaç OF, Başkaya HS. Kirlenmiş toprakların biyoremediasyon ile ıslahı. Uludağ Üniversitesi Mühendislik-Mimarlık Fakültesi Dergisi. 2010;2(15): 123–137.
Tekere M. Microbial Bioremediation and Different Bioreactors Designs Applied. In: Jacob -Lopes E, Queiroz Zepka L (eds.) Biotechnology and Bioengineering. IntechOpen; 2019. p. 1–14. https://doi.org/10.5772/INTECHOPEN.83661.
Sharma I. Bioremediation Techniques for Polluted Environment: Concept, Advantages, Limitations, and Prospects. In: Murillo-Tovar MA, Saldarriaga-Noreña H, Saeid A (eds.) Trace Metals in the Environment - New Approaches and Recent Advances. IntechOpen; 2020. https://doi.org/10.5772/INTECHOPEN.90453.
Alori ET, Gabasawa AI, Elenwo CE, Agbeyegbe OO. Bioremediation techniques as affected by limiting factors in soil environment. Frontiers in Soil Science. 2022;2: 937186. https://doi.org/10.3389/FSOIL.2022.937186/BIBTEX.
Gürgan M, İrez Eİ, Adiloğlu S. Understanding Bioremediation of Metals and Metalloids by Genomic Approaches. Omics Insights in Environmental Bioremediation. 2022; 375–392. https://doi.org/10.1007/978-981-19-4320-1_16.
Mousavi SM, Hashemi SA, Iman Moezzi SM, Ravan N, Gholami A, Lai CW, et al. Recent Advances in Enzymes for the Bioremediation of Pollutants. Biochemistry Research International. 2021;2021(1): 5599204. https://doi.org/10.1155/2021/5599204.
Bhandari S, Poudel DK, Marahatha R, Dawadi S, Khadayat K, Phuyal S, et al. Microbial Enzymes Used in Bioremediation. Journal of Chemistry. 2021;2021(1): 8849512. https://doi.org/10.1155/2021/8849512.
Aislabie J, Lloyd-Jones G. A review of bacterial-degradation of pesticides. Australian Journal of Soil Research. 1995;33(6): 925–942. https://doi.org/10.1071/SR9950925.
Huang Y, Xiao L, Li F, Xiao M, Lin D, Long X, et al. Microbial Degradation of Pesticide Residues and an Emphasis on the Degradation of Cypermethrin and 3-phenoxy Benzoic Acid: A Review. Molecules 2018, Vol. 23,. 2018;23(9). https://doi.org/10.3390/MOLECULES23092313.
Kopittke PM, Harper SM, Asio LG, Asio VB, Batalon JT, Batuigas AMT, et al. Soil degradation: An integrated model of the causes and drivers. International Soil and Water Conservation Research. 2025. p. 744–755. https://doi.org/10.1016/j.iswcr.2025.07.010.
Abubakar Y, Tijjani H, Egbuna C, Adetunji CO, Kala S, Kryeziu TL, et al. Pesticides, History, and Classification. Natural Remedies for Pest, Disease and Weed Control. 2020; 29–42. https://doi.org/10.1016/B978-0-12-819304-4.00003-8.
Özercan B, Taşçı R. Investigation of Pesticide Use in Türkiye in Terms of Provinces, Regions and Pesticide Groups. Ziraat Mühendisliği. 2022;(375): 75–88. https://doi.org/10.33724/zm.1120599.
Zhang W. Global pesticide use: Profile, trend, cost / benefit and more. Proceedings of the International Academy of Ecology and Environmental Sciences. 2018. www.iaees.org
Wachowska U, Kucharska K, Pluskota W, Czaplicki S, Stuper-Szablewska K. Bacteria Associated with Winter Wheat Degrade Fusarium Mycotoxins and Triazole Fungicide Residues. Agronomy 2020, Vol. 10,. 2020;10(11). https://doi.org/10.3390/AGRONOMY10111673.
Perruchon C, Pantoleon A, Veroutis D, Gallego-Blanco S, Martin-Laurent F, Liadaki K, et al. Characterization of the biodegradation, bioremediation and detoxification capacity of a bacterial consortium able to degrade the fungicide thiabendazole. Biodegradation 2017 28:5. 2017;28(5): 383–394. https://doi.org/10.1007/S10532-017-9803-Z.
Howell CC, Semple KT, Bending GD. Isolation and characterisation of azoxystrobin degrading bacteria from soil. Chemosphere. 2014;95: 370–378. https://doi.org/10.1016/J.CHEMOSPHERE.2013.09.048.
Şahin N, Tamer AÜ. Isolation, Characterization and Identification ofThiram-degrading Microorganisms from SoilEnrichment Cultures. Turkish Journal of Biology. 2000;24(2): 353–364.
Cech JS, Hartman P, Slosárek M, Chudoba J. Isolation and identification of a morpholine-degrading bacterium. Applied and environmental microbiology. 1988;54(2): 619–621. https://doi.org/10.1128/AEM.54.2.619-621.1988
Poupin P, Truffaut N, Combourieu B, Besse P, Sancelme M, Veschambre H, et al. Degradation of morpholine by an environmental Mycobacterium strain involves a cytochrome P-450. Applied and Environmental Microbiology. 1998;64(1): 159–165. https://doi.org/10.1128/AEM.64.1.159-165.1998
Russell PE. A century of fungicide evolution. The Journal of Agricultural Science. 2005;143(1): 11–25. https://doi.org/10.1017/S0021859605004971.
Zhang C, Li J, Wu X, Long Y, An H, Pan X, et al. Rapid degradation of dimethomorph in polluted water and soil by Bacillus cereus WL08 immobilized on bamboo charcoal–sodium alginate. Journal of Hazardous Materials. 2020;398: 122806. https://doi.org/10.1016/J.JHAZMAT.2020.122806.
Sharma R, Walia A, Putatunda C, Solanki P. Impact of pesticides on microbial diversity. Current Developments in Biotechnology and Bioengineering: Pesticides: Human Health, Environmental Impacts and Management. 2023; 427–458. https://doi.org/10.1016/B978-0-323-91900-5.00001-1.
Streletskii R, Astaykina A, Krasnov G, Gorbatov V. Changes in Bacterial and Fungal Community of Soil under Treatment of Pesticides. Agronomy 2022, Vol. 12,. 2022;12(1). https://doi.org/10.3390/AGRONOMY12010124.
Mongodin EF, Shapir N, Daugherty SC, DeBoy RT, Emerson JB, Shvartzbeyn A, et al. Secrets of Soil Survival Revealed by the Genome Sequence of Arthrobacter aurescens TC1. PLOS Genetics. 2006;2(12): e214. https://doi.org/10.1371/JOURNAL.PGEN.0020214.
Albers P, Weytjens B, De Mot R, Marchal K, Springael D. Molecular processes underlying synergistic linuron mineralization in a triple-species bacterial consortium biofilm revealed by differential transcriptomics. MicrobiologyOpen. 2018;7(2). https://doi.org/10.1002/mbo3.559.
Gürgan M, İrez Eİ, Adiloğlu S. Understanding Bioremediation of Metals and Metalloids by Genomic Approaches. In: Kumar V, Thakur IS (eds.) Omics Insights in Environmental Bioremediation. Springer, Singapore; 2022. p. 375–392. https://doi.org/10.1007/978-981-19-4320-1_16.
Olaniran AO, Balgobind A, Pillay B. Bioavailability of Heavy Metals in Soil: Impact on Microbial Biodegradation of Organic Compounds and Possible Improvement Strategies. International Journal of Molecular Sciences 2013, Vol. 14, Pages 10197-10228. 2013;14(5): 10197–10228. https://doi.org/10.3390/IJMS140510197.
Jayaram S, Ayyasamy PM, Aiswarya KP, Devi MP, Rajakumar S. Mechanism of Microbial Detoxification of Heavy Metals: A Review. Journal of Pure and Applied Microbiology. 2022. p. 1562–1574. https://doi.org/10.22207/JPAM.16.3.64.
Osinuga OA, Aduloju AB, Oyegoke CO. Impact of agrochemicals application on soil quality indicators and trace elements level of wetlands under different uses. Journal of Trace Elements and Minerals. 2023;5: 100090. https://doi.org/10.1016/J.JTEMIN.2023.100090.
Yin K, Lv M, Wang Q, Wu Y, Liao C, Zhang W, et al. Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1. Water Research. 2016;103: 383–390. https://doi.org/10.1016/J.WATRES.2016.07.053.
Chang J ‐S, Hong J. Biosorption of mercury by the inactivated cells of pseudomonas aeruginosa PU21 (Rip64). Biotechnology and Bioengineering. 1994;44(8): 999–1006. https://doi.org/10.1002/BIT.260440817
Andreazza R, Pieniz S, Okeke BC, Camargo FAO. Evaluation of copper resistant bacteria from vineyard soils and mining waste for copper biosorption. Brazilian Journal of Microbiology. 2011;42(1): 66–74. https://doi.org/10.1590/S1517-83822011000100009.
Atuchin V V., Asyakina LK, Serazetdinova YR, Frolova AS, Velichkovich NS, Prosekov AY. Microorganisms for Bioremediation of Soils Contaminated with Heavy Metals. Microorganisms 2023, Vol. 11,. 2023;11(4). https://doi.org/10.3390/MICROORGANISMS11040864.
Wu R, Yao F, Li X, Shi C, Zang X, Shu X, et al. Manganese Pollution and Its Remediation: A Review of Biological Removal and Promising Combination Strategies. Microorganisms 2022, Vol. 10,. 2022;10(12). https://doi.org/10.3390/MICROORGANISMS10122411.
Gul I, Adil M, Lv F, Li T, Chen Y, Lu H, et al. Microbial strategies for lead remediation in agricultural soils and wastewater: mechanisms, applications, and future directions. Frontiers in Microbiology. 2024;15: 1434921. https://doi.org/10.3389/FMICB.2024.1434921
