Mikroçoğaltım ve Hastalıksız Bitki Üretimi

Abdalla, N., El-Ramady, H., Seliem, M. K., El-Mahrouk, M. E., Taha, N., Bayoumi, Y., Shalaby, T. A., Dobránszki, J., 2022. An academic and technical overview on plant micropropagation challenges. Horticulturae, 8, 677. https://doi.org/10.3390/horticulturae8080677

Abido, A. I. A., Aly, M. A. M., Hassanen, S. A., Rayan, G. A., 2013. In vitro propagation of grapevine (Vitis vinifera L.) Muscat of Alexandria cv. for conservation of endangerment. Middle-East Journal of Scientific Research, 13(3), 328-337. https://doi.org/10.5829/idosi.mejsr.2013.13.3.1926

Adil, S., Singh, V., Anjum, A., Quraishi, A., 2022. A mini-review on electrotherapeutic strategy for the plant viral elimination. Plant Cell, Tissue and Organ Culture (PCTOC), 150, 41-55. https://doi.org/10.1007/s11240-022-02265-w

Anonim, (2025). Mikro çoğaltım [Ders notu]. Ankara Üniversitesi Açık Ders Malzemeleri. Erişim adresi: https://acikders.ankara.edu.tr/mod/resource/view.php?id=89500

Ahmad, N., Fatima, N., Faisal, M., Alatar, A. A., Pathirana, R., 2022. Photosynthetic parameters and oxidative stress during acclimation of crepe-myrtle (Lagerstroemia speciosa (L.) Pers.) in a meta-topolin-based micropropagation system and genetic fidelity of regenerated plants. Plants, 11(9), 1163. https://doi.org/10.3390/plants11091163

Ajithkumar, D., Seeni, S., 1998. Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L.) Corr., a medicinal tree. Plant Cell Rep., 17(5), 422-426. https://doi.org/10.1007/s002990050418

Al-Taleb, M. M., Hassawi, D. S., Abu-Romman, S. M., 2011. Production of virus free potato plants using meristem culture from cultivars grown under Jordanian environment. American-Eurasian J. Agric. & Environ. Sci., 11(4), 467-472.

Bahri, N. B., Bettaieb, T., 2013. In vitro propagation of a forest tree Paulownia tomentosa (Thunb.) Steud.-A valuable medicinal tree species. Albanian Journal of Agricultural Sciences, 12(1), 37-42.

Barton, M. K., 2010. Twenty years on: the inner workings of the shoot apical meristem, a developmental dynamo. Developmental Biology, 341(1), 95-113. https://doi.org/10.1016/j.ydbio.2009.11.029

Basile, G. N., Tedone, L., Pulvento, C., De Mastro, G., Ruta, C., 2023. Establishment of an efficient in vitro propagation protocol for Cannabis sativa L. subsp. ruderalis Janish. Horticulturae, 9(11), 1241. https://doi.org/10.3390/horticulturae9111241

Benke, A. P., Krishna, R., Khandagale, K., Gawande, S., Shelke, P., Dukare, S., Dhumal, S., Singh, M., Mahajan, V., 2023. Efficient elimination of viruses from garlic using a combination of shoot meristem culture, thermotherapy, and chemical treatment. Pathogens, 12(1), 129. https://doi.org/10.3390/pathogens12010129

Bharat, J. S., Nandkishor, Z. V., Natha, T. S., Avinash, S. A., 2020. Plant tissue culture. Journal of Emerging Technologies and Innovative Research, 7(11), 229-239.

Bhat, A. I., Rao, G. P., 2020. Virus Elimination by Meristem-Tip Culture. In: Characterization of Plant Viruses . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0334-5_47

Bi, W. L., Hao, X. Y., Cui, Z. H., Pathirana, R., Volk, G. M., Wang, Q. C., 2018. Shoot tip cryotherapy for efficient eradication of grapevine leafroll-associated virus-3 from diseased grapevine in vitro plants. Annals of Applied Biology, 173(3), 261-270. https://doi.org/10.1111/aab.12459

Brison, M., de Boucaud, M. T., Pierronnet, A., Dosba, F., 1997. Effect of cryopreservation on the sanitary state of a cv Prunus rootstock experimentally contaminated with Plum Pox Potyvirus. Plant Science, 123(1-2), 189-196. https://doi.org/10.1016/S0168-9452(97)04581-0

Bonnart, R. M., Chen, K. Y., Volk, G. M., 2022. Plant Tissue Culture Media Preparation. In: Volk G. M. (eds.) Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. https://colostate.pressbooks.pub/clonalcryopreservation/chapter/media/

Campol, J. R., Naing, A. H., Aung, H. M., Cho, S. B., Kang, H., Chung, M. Y., Kim, C. K., 2024. Production of genetically stable and Odontoglossum ringspot virus-free Cymbidium orchid ‘New True’plants via meristem-derived protocorm-like body (PLB) subcultures. Plant Methods, 20(1), 145. https://doi.org/10.1186/s13007-024-01269-1

Comlekcioglu, S., Kuden, A. B., Kacar, Y. A., Kamberoglu, M. A., 2007. Meristem culture of two fig cultivars in Turkey. Fruits, 62(2), 125-131. https://doi.org/10.1051/fruits:2007006

Conde, F., Carmona-Martin, E., Hormaza, J. I., Petri, C., 2023. In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes. In Vitro Cell. Dev. Biol.-Plant, 59(2), 197-208. https://doi.org/10.1007/s11627-023-10334-8

Cox, K., 2024. Temperature and light in tissue-cultured plant success. The Regular Plant Co. https://theregularplantco.com/temperature-and-light-in-tissue-cultured-plant-success/

Diab, A. A., Khalil, S. M., Ismail, R. M., 2011. Regeneration and micropropagation of grapevine (Vitis vinifera L.) through shoot tips and axillary buds. International Journal of Advanced Biotechnology and Research, 2(4), 484-491.

Disasa, T., Feyissa, T., Dagne, K., 2011. In vitro regeneration of niger (Guizotia abyssinica L.F.) Cass.). Int. J. Biosci., 1(6), 110-118.

Doğan, M., 2019. Zeatin ve Farklı Oksin Kombinasyonlarının Önemli Tıbbi Bitki Limnophila aromatica (Lamk.) Merr.’nın In Vitro Mikroçoğaltımı Üzerine Etkisi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 22, 323-329. https://doi.org/10.18016/ksutarimdoga.vi.555790

Dolatabadi, K., Davarynejad, G., Safarnejad, M. R., Ghayoor, Z., 2023. Developing virus-free grapevine explants by using silver-nanoparticles and its comparison with chemo and thermotherapy-based approaches. Journal of Crop Protection, 12(1), 15-27. https://doi.org/10.48311/jcp.2023.1618

Ebrahimi, M., Habashi, A. A., Emadpour, M., Kazemi, N., 2022. Recovery of virus-free Almond (Prunus dulcis) cultivars by somatic embryogenesis from meristem undergone thermotherapy. Scientific Reports, 12(1), 14948. https://doi.org/10.1038/s41598-022-19269-3

Espinosa-Leal, C. A., Puente-Garza, C. A., García-Lara, S., 2018. In vitro plant tissue culture: means for production of biological active compounds. Planta, 248(1), 1-18. https://doi.org/10.1007/s00425-018-2910-1

Fouracre, J. P., Poethig, R. S., 2020. Lonely at the top? Regulation of shoot apical meristem activity by intrinsic and extrinsic factors. Curr. Opin. Plant Biol., 58, 17-24. https://doi.org/10.1016/j.pbi.2020.08.008

García-Gonzáles, R., Quiroz, K., Carrasco, B., Caligari, P., 2010. Plant tissue culture: Current status, opportunities and challenges. Cien. Inv. Agr., 37(3), 5-30.

Gaj, M. D., 2004. Factors influencing somatic embryogenesis induction and plant regeneration with particular reference to Arabidopsis thaliana (L.) Heynh. Plant Growth Regulation, 43(1), 27-47.

George, E. F., Hall, M. A., De Klerk, G. J. (eds.)., 2008. Plant propagation by tissue culture, 3rd ed.. Springer. https://doi.org/10.1007/978-1-4020-5005-3

Gulzar, B., Mujib, A., Malik, M. Q., Mamgain, J., Syeed, R., Zafar, N., 2020. Plant tissue culture: agriculture and industrial applications. In: Transgenic Technology Based Value Addition in Plant Biotechnology (pp. 25-49). Academic Press. https://doi.org/10.1016/B978-0-12-818632-9.00002-2

Gupta, N., Jain, V., Joseph, M. R., Devi, S., 2020. A review on micropropagation culture method. Asian Journal of Pharmaceutical Research and Development, 8(1), 86-93. https://doi.org/10.22270/ajprd.v8i1.653

Gümüş, C., Ellialtıoğlu, Ş. Ş., 2018. Kalanchoe blossfeldiana Poelln. Türünde Yapılan Doku Kültürü Araştırmaları Üzerinde Bir İnceleme. Türk Bilimsel Derlemeler Dergisi, 11(1), 18-26.

Haberlandt, G., 1902. Kulturversuche mit isolierten Pflanzenzellen. Sitzungsber. Akad. Wiss. Wien. Math.-Naturwiss. Kl. Abt. J. 111, 69-92.

Hasnain, A., Naqvi, S. A. H., Ayesha, S. I., Khalid, F., Ellahi, M., Iqbal, S., Hassan, M. Z., Abbas, A., Adamski, R., Markowska, D., Baazeem, A., Mustafa, G., Moustafa, M., Hasan, M. E., Abdelhamid, M. M. A., 2022. Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches. Frontiers in Plant Science, 13, 1009395. https://doi.org/10.3389/fpls.2022.1009395

Herman, E .B., 2015. Media and techniques for growth, regeneration and storage: 2011–2015. In: Lake, M. (ed.). Recent advances in plant tissue culture.Agritech Consultants Inc., Shrub Oak, NY, p. 148.

Hormozi-Nejad, M. H., Mozafari, J., Rakhshandehroo, F., 2010. Elimination of Bean common mosaic virus using an electrotherapy technique. J. Plant Dis. Prot., 117(5), 201-205. https://doi.org/10.1007/BF03356361

Hu, G. J., Hong, N., Wang, L. P., Hu, H. J., Wang, G. P., 2012. Efficacy of virus elimination from in vitro-cultured sand pear (Pyrus pyrifolia) by chemotherapy combined with thermotherapy. Crop Protection, 37, 20-25. https://doi.org/10.1016/j.cropro.2012.02.017

Hu, G., Dong, Y., Zhang, Z., Fan, X., Ren, F., 2020. Efficiency of chemotherapy combined with thermotherapy for eliminating Grapevine leafroll-associated virus 3 (GLRaV-3). Sci. Hortic. 271, 109462 https://doi.org/10.1016/j.scienta.2020.109462

James, D., Trytten, P. A., Mackenzie, D. J., Towers, G. H. N., French, C. J., 1997. Elimination of Apple stem grooving virus by chemotherapy and development of an immunocapture RT-PCR for rapid sensitive screening. Annals of Applied Biology, 131(3), 459-470. https://doi.org/10.1111/j.1744-7348.1997.tb05173.x

Jakab, Z. I., Vlașin, L. D., Chiorean, A. M., 2022. Behaviour of several chokeberry cultivars (Aronia melanocarpa) at the in vitro micropropagation. Romanian Journal of Horticulture, 3, 31-36. https://doi.org/10.51258/RJH.2022.04

Jiménez, V. M., 2005. Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regulation, 47(2), 91-110. https://doi.org/10.1007/s10725-005-3478-x

Justine, A. K., Kaur, N., Savita, Pati, P. K., 2022. Biotechnological interventions in banana: current knowledge and future prospects. Heliyon, 8(11), e11636. https://doi.org/10.1016/j.heliyon.2022.e11636

Kabir, A. H., Pal, S. P., Sarker, K. K., Sharmin, S. A., Alam, M. F., 2010. Virus elimination and pathogen-free plantlets regeneration in Cucurbita pepo L. Archives of Phytopathology and Plant Protection, 43(6), 527-537.

Kassaye, E., Bekele, B. D., 2015. In vitro optimization of the protocol for micropropagation of plum (Prunus salicina l. var. Methley) from nodal explants. Biotechnology International, 8(4), 137-148.

Kaur, K., Dolker, D., Behera, S., Pati, P. K., 2022. Critical factors influencing in vitro propagation and modulation of important secondary metabolites in Withania somnifera (L.) dunal. Plant Cell Tiss. Organ Cult., 149(1), 41-60. https://doi.org/10.1007/s11240-021-02225-w

Kaviani, B., 2015. Some Useful Information about Micropropagation. Journal of Ornamental & Horticultural Plants, 5(1), 29-40.

Kaviani, B., Deltalab, B., Kulus, D., Tymoszuk, A., Bagheri, H., Azarinejad, T., 2022. In Vitro Propagation of Pyracantha angustifolia (Franch.) CK Schneid. Horticulturae, 8(10), 964. https://doi.org/10.3390/horticulturae8100964

Kaya, E., 2021. Comparison of three different techniques for eradication of Apple mosaic virus (ApMV) from hazelnut (Corylus avellana L.). Journal of Plant Protection Research. 61(1), 11-19. https://doi.org/10.24425/jppr.2021.136275

Kaynak, L., Ersoy, N., 1997. Bitki büyüme düzenleyicilerinin genel özellikleri ve kullanım alanları. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 10, 223-236.

Kaynak, L., İmamgiller, B., 1997. Bitki büyüme düzenleyicilerinin f izyolojik olaylardaki rolleri. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 10, 289-299.

Kaynak, L., Memiş, M., 1997. Bitki büyüme engelleyici ve geciktiricilerinin etki mekanizmaları. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 10, 237-248.

Kazemi, N., Zaare Nahandi, F., Habashi, A. A., Masoomi-Aladizgeh, F., 2020. Comparing the efficiency of conventional and novel methods of virus elimination using molecular techniques. Eur. J. Plant Pathol., 157(4), 887-897. https://doi.org/10.1007/s10658-020-02048-z

Kim, M. Y., Cho, K. H., Chun, J. A., Park, S. J., Kim, S. H., Lee, H. C., 2017. Elimination of Grapevine fleck virus from infected grapevines ‘Kyoho’through meristem-tip culture of dormant buds. Journal of Plant Biotechnology, 44(4), 401-408.

Köhl, J., Kolnaar, R., Ravensberg, W. J., 2019. Mode of action of microbial biological control agents against plant diseases: Relevance beyond efficacy. Front. Plant Sci., 10, 845. https://doi.org/10.3389/fpls.2019.00845

Kulus, D., Tymoszuk, A., 2024. Advancements in in vitro technology: A comprehensive exploration of micropropagated plants. Horticulturae, 10(1), 88. https://doi.org/10.3390/horticulturae10010088

Kumar, S., Khan, M. S., Raj, S. K., Sharma, A. K., 2009. Elimination of mixed infection of Cucumber mosaic and Tomato aspermy virus from Chrysanthemum morifolium Ramat. cv. Pooja by shoot meristem culture. Sci. Hortic., 119(2), 108-112. https://doi.org/10.1016/j.scienta.2008.07.017

Kumar, A., Bhuj,B. D., Dhar, S., Dixit, K. M. J., Singh, S. P., 2023. Micropropagation of Fruit Crops: A Review. Adv. Crop Sci. Tech., 11, 595.

Kumlay, A. M., Eryiğit, T., 2011. Bitkilerde Büyüme ve Gelişmeyi Düzenleyici Maddeler: Bitki Hormonları. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 1(2), 47-56.

Lal, M., Jamwal, M., Sood, Y., Bakshi, P., Sharma, N., Sharma, S., Kumar, S., 2023. Micropropagation of fruit crops: A review. Plant Science Today, 10(1), 108-117. https://doi.org/10.14719/pst.1891

Ling, W. T., Tan, L. V., Khor, S. P., Sriskanda, D., Subramaniam, S., Chew, B. L., 2022. Rapid in vitro propagation of fig (Ficus carica L.)‘Violette de Solliès’ supported by molecular and microscopy analyses. Horticulturae, 8(11), 1025. https://doi.org/10.3390/horticulturae8111025

Maheshwari, R., 1969. Applications of plant tissue and cell culture in the study of physiology of parasitism. Proc. Indian Acad. Sci. 69, 152-172. https://doi.org/10.1007/BF03052523

Marino, L.A., Ruffa, P., Mozzanini, E., Patono, D. L., Sereno, A., Pavese, V., 2025. LEDs in Plant Tissue Culture: Boosting Micropropagation of Castanea sativa Cultivars. J. Plant Growth Regul., 44, 6046–6060. https://doi.org/10.1007/s00344-025-11812-6

Mazeikiene, I., Kviklys, D., Siksnianiene, J. B., Zinkus, D., Stanys, V., 2019. Influence of Ribavirin on Prunus domestica L. regeneration, genome stability and virus eradication in vitro. Proceedings of the Latvian Academy of Sciences,73(3), 238-243. https://doi.org/10.2478/prolas-2019-0038

Meira, F. S., Shao, M., Niedz, R. P., Thomson, J. G., 2024. Evaluation of 21 different media on shoot regeneration in 11 cultivars of citrus using juvenile tissue. Plant Cell Tiss. Organ Cult., 158, 8. https://doi.org/10.1007/s11240-024-02785-7

Meybodi, D. E., Mozafari, J., Babaeiyan, N., Rahimian, H., 2011. Application of electrotherapy for the elimination of potato potyviruses. J. Agr. Sci. Tech., 13, 921-927.

Muñoz-Concha, D., Quintero, J., Ercişli, S., 2021. Media and hormones influence in micropropagation success of blackberry cv.‘Chester’. Res. J. Biotechnol., 16, 5.

Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant., 15, 473-497.

Muhammad, A., Rashid, H., Hussain, I., Naqvi, S. S., 2007. Proliferation-rate Effects of BAP and Kinetin on Banana (Musa spp. AAA Group) ‘Basrai’. HortScience, 42(5), 1253-1255. https://doi.org/10.21273/HORTSCI.42.5.1253

Mostafa, F. M. A., Shaaban, M. M., Elazab, D. S., Kamel, M. T., 2015. In vitro Propagation of Four Grape Cultivars. Assiut Journal of Agricultural Sciences, 46(4), 65-76.

Nongdam, P., Beleski, D. G., Tikendra, L., Dey, A., Varte, V., El Merzougui, S., Pereira, V. M., Barros, P. R., Vendrame, W. A., 2023. Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review. Plants, 12(5), 1136. https://doi.org/10.3390/plants12051136

Neri, J. C., Meléndez-Mori, J. B., Tejada-Alvarado, J. J., Vilca-Valqui, N. C., Huaman-Huaman, E., Oliva, M., Goñas, M., 2022. An optimized protocol for micropropagation and acclimatization of strawberry (Fragaria× ananassa Duch.) variety ‘Aroma’. Agronomy, 12(4), 968. https://doi.org/10.3390/agronomy12040968

Panattoni, A., Luvisi, A., Triolo, E., 2013. Elimination of viruses in plants: twenty years of progress. Span. J. Agri Res., 11, 173-188. https://doi.org/10.5424/sjar/2013111- 3201

Papafotiou, M., Vlachou, G., Martini, A. N., 2023. Investigation of the effects of the explant type and different plant growth regulators on micropropagation of five mediterranean Salvia spp. native to Greece. Horticulturae, 9(1), 96. https://doi.org/10.3390/horticulturae9010096

Pasternak, T. P., Steinmacher, D., 2024. Plant Growth Regulation in Cell and Tissue Culture In Vitro. Plants, 13(2), 327. https://doi.org/10.3390/plants13020327

Phillips, G. C., Garda, M., 2019. Plant tissue culture media and practices: an overview. In Vitro Cell. Dev. Biol.-Plant, 55, 242-257. https://doi.org/10.1007/s11627-019-09983-5

Pourhassan, A., Kaviani, B., Kulus, D., Miler, N., Negahdar, N., 2023. A complete micropropagation protocol for black-leaved Zamioculcas zamiifolia (Lodd.) Engl.‘Dowon’. Horticulturae, 9(4), 422. https://doi.org/10.3390/horticulturae9040422

Quiroz, K. A., Berríos, M., Carrasco, B., Retamales, J. B., Caligari, P. D. S., García-Gonzáles, R., 2017. Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.). Biol. Res., 50, 20. https://doi.org/10.1186/s40659-017-0125-8

Raj, R., Kaur, C., Agrawal, L., Kumar, S., Chauhan, P. S., Raj, S. K., 2022. Development of a protocol for the elimination of Cyrtanthus elatus virus-A from Narcissus tazetta by in vitro chemotherapy in combination with electrotherapy. J. Virol. Methods, 300, 114368. https://doi.org/10.1016/j.jviromet.2021.114368

Ranaweera, K. K., Gunasekara, M. T. K., Eeswara, J. P., 2013. Ex vitro rooting: A low cost micropropagation technique for Tea (Camellia sinensis (L.) O. kuntze) hybrids. Scientia Horticulturae, 155, 8-14. https://doi.org/10.1016/j.scienta.2013.03.001

Sarıdaş, M. A., Baktemur, G., Taşkın, H., Kargı, S. P., 2021. Effect of plant hormones on micropropagation potential of superior strawberry genotypes and their parents via shoot-tip culture. Acta Sci. Pol. Hortorum Cultus, 20(3), 63-75. https://doi.org/10.24326/asphc.2021.3.7

Sarropoulou, V., Grigoriadou, K., Maliogka, V. I., Sassalou, C. L., Ziogas, V., 2024. The Elimination of Viroids through In vitro Thermotherapy and a Meristem Tip Culture from a New Limonime Hybrid (Citrus x limon var. limon (L.) Burm. f. x Citrus latifolia var. latifolia). BioTech, 13(3), 37. https://doi.org/10.3390/biotech13030037

Schwach, F., Vaistij, F. E., Jones, L., Baulcombe, D. C., 2005. An RNA-dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal. Plant Physiol., 138(4), 1842-1852. https://doi.org/10.1104/pp.105.063537

Shahzad, A., Parveen, S., Sharma, S., Shaheen, A., Saeed, T., Yadav, V., Akhtar, R., Ahmad, Z., Upadhyay, A., 2017. Plant tissue culture: applications in plant improvement and conservation. In: Plant Biotechnology: Principles and Applications (pp. 37-72). Singapore: Springer Singapore.

Sharma, A. T., Jadhav, D. S., Dachawar, R. R., Pawde, D. V., Zunzulde, S. S., 2024. Plant Tissue Culture: A Review. International Journal of Pharmacy and Pharmaceutical Research, 30(5), 595-612.

Singh, A., 2015. Micropropagation of plants. In: Plant Biology And Biotechnology: Volume II: Plant Genomics And Biotechnology (pp. 329-346). New Delhi: Springer India.

Singh, V., Adil, S., Quraishi, A., 2022. Elimination of BBTV via a systemic in vitro electrotherapy approach. Journal of Virological Methods, 300, 114367. https://doi.org/10.1016/j.jviromet.2021.114367

Sultana, K. W., Das, S., Chandra, I., Roy, A., 2022. Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers. Sci. Rep., 12(1), 1683. https://doi.org/10.1038/s41598-022-05787-7

Szabó, L. K., Desiderio, F., Kirilla, Z., Hegedűs, A., Várallyay, É., Preininger, É., 2024. Elimination of Cherry virus A from Prunus domestica ‘Besztercei Bt. 2’using in vitro techniques. Plant Cell Tiss. Organ Cult., 157, 45. https://doi.org/10.1007/s11240-024-02770-0

Taşkın, H., Baktemur, G., Kurul, M., Büyükalaca, S., 2013. Use of tissue culture techniques for producing virus-free plant in garlic and their identification through real-time PCR. The Scientific World Journal, 2013(1), 781282. https://doi.org/10.1155/2013/781282

Thanuja, K., Arulmozhiyan, R., Saraswathi, M. S., Selvarajan, R., Jegadeeswari, V., Rajanbabu, V., 2025. A comprehensive review on in vitro therapies for virus elimination and novel methods for virus protection in key horticultural crops. Planta, 262(1), 15. https://doi.org/10.1007/s00425-025-04718-w

VanDerZanden, A. M., 2012. How hormones and growth regulators affect your plants. OSU Extension Service. https://extension.oregonstate.edu/gardening/techniques/how-hormones-growth-regulators-affect-your-plants

Vivek, M., Modgil, M., 2018. Elimination of viruses through thermotherapy and meristem culture in apple cultivar ‘Oregon Spur-II’. Virus Disease, 29(1), 75-82. https://doi.org/10.1007/s13337-018-0437-5

Yan, H.B., Liang, C. X., Yang, L. T., Li, Y. R., 2010. In vitro and ex vitro rooting of Siratia grosvenorii, a traditional medicinal plant. Acta Physiol. Plant, 32, 115-120. https://doi.org/10.1007/s11738-009-0386-0

Walsh, C. S., 2003. Plant Hormones. Concise Encyclopedia of Temperate Tree Fruit. In: Singha, S., Baugher T (eds.). and, Haworth Press. ISBN 1560229411.

Wang, Q., Cuellar, W. J., Rajamäki, M. L., Hirata, Y., Valkonen, J. P., 2008. Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Mol. Plant Pathol., 9(2), 237-250. https://doi.org/10.1111/j.1364-3703.2007.00456.x

Wang, Q. C., Valkonen, J. P. T., 2008. Efficient elimination of sweetpotato little leaf phytoplasma from sweetpotato by cryotherapy of shoot tips. Plant Pathology, 57(2), 338-347. https://doi.org/10.1111/j.1365-3059.2007.01710.x

Wang, Q., Valkonen, J. P., 2009. Cryotherapy of shoot tips: novel pathogen eradication method. Trends Plant Sci., 14, 119-122. https://doi.org/10.1016/j. tplants.2008.11.010

Wang, Q. C., Panis, B., Engelmann, F., Lambardi, M., Valkonen, J. P. T., 2009. Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation. Annals of Applied Biology, 154(3), 351-363. https://doi.org/10.1111/j.1744-7348.2008.00308.x

Wang, M. B., Masuta, C., Smith, N. A., Shimura, H., 2012. RNA silencing and plant viral diseases. Mol. Plant Microbe Interact., 25(10), 1275-1285. https://doi.org/10.1094/MPMI-04-12-0093-CR

Wang, M. R., Cui, Z. H., Li, J. W., Hao, X. Y., Zhao, L., Wang, Q. C., 2018. In vitro thermotherapy-based methods for plant virus eradication. Plant Methods, 14, 87. https://doi.org/10.1186/s13007-018-0355-y

Waseem, K., Jilani, M. S., Khan, M. S., 2009. Rapid plant regeneration of chrysanthemum (Chrysanthemum morifolium L.) through shoot tip culture. African Journal of Biotechnology, 8(9), 1871-1877.

Wöhner, T., Höfer, M., 2024. Evaluation of cryotherapy and meristem isolation from stolons to eliminate viruses in Fragaria germplasm. J. Plant Pathol., 106, 729-735. https://doi.org/10.1007/s42161-023-01570-3

Zarbakhsh, S., Shahsavar, A. R., Soltani, M., 2024. Optimizing PGRs for in vitro shoot proliferation of pomegranate with bayesian-tuned ensemble stacking regression and NSGA-II: a comparative evaluation of machine learning models. Plant Methods, 20(1), 82. https://doi.org/10.1186/s13007-024-01211-5

Zhang, A. L., Bettoni, J. C., Shi, X., Liu, Y., Yang, B., Liu, Z., 2024. In vitro chemotherapy based methods for virus elimination from Actinidia macrosperma. Sci. Hortic., 337, 113543. https://doi.org/10.1016/j.scienta.2024.