Protein Değerlendirme Sistemleri

Özet

Protein, hayvan beslemede büyüme, üreme, metabolik faaliyetlerin sürdürülmesi ve verimliliğin artırılması için olmazsa olmaz, bir besin öğesidir. Rasyondaki proteinin doğru değerlendirilmesi, besin maddelerinin etkin kullanımını sağlamak ve azot kayıplarını en aza indirmek açısından büyük önem taşır. Klasik ham protein (HP) analizi, yalnızca toplam azot miktarını belirlemektedir ve amino asitlerin biyolojik değeri veya sindirilebilirliği hakkında bilgi vermemektedir. Buna karşılık, gerçek protein (GP), sindirilebilir protein ve amino asit temelli sistemler, hayvan tarafından kullanılabilir gerçek protein miktarının daha doğru biçimde değerlendirilmesine olanak tanır. Tek mideli hayvanlarda protein kalitesi büyük ölçüde amino asit profilinin dengesi ve sindirilebilirliğine bağlıdır; lizin, metiyonin ve treonin gibi sınırlayıcı amino asitlerin düzeyleri büyüme ve yemden yararlanma performansını doğrudan etkiler. Ruminantlarda ise rumende parçalanabilir protein (RDP), mikrobiyal protein sentezi için temel azot kaynağı iken, rumende parçalanamayan protein (RUP) rumenden geçerek ince bağırsakta sindirilir. Ruminantlarda metabolize olabilir protein (MP) arzını mikrobiyal protein ve RUP’un sindirilebilen kısmının toplamı belirler. Mikrobiyal protein üretiminde fermente olabilir enerji ile rumende parçalanabilir azot arasındaki dengenin sağlanması gerekmektedir. Bu nedenle protein değerlendirme sistemleri, yalnızca azot miktarını değil; mikrobiyal protein sentezi, amino asit kullanılabilirliği ve post-ruminal sindirilebilirlik gibi faktörleri de bütüncül biçimde dikkate almalıdır. Bu kitap bölümünde, proteinin değerlendirilmesinde kullanılan kimyasal, in vitro ve in vivo yöntemler derlenmiş; bu sistemlerin tek mideli ve ruminant hayvan beslemesindeki uygulamaları tartışılmıştır. Bu bütüncül yaklaşım sayesinde, protein kullanım etkinliğini artırmak, hayvan performansını iyileştirmek ve çevresel azot kayıplarını azaltmak suretiyle sürdürülebilir hayvansal üretimi desteklenebilir.

Referanslar

Alhotan RA, Pesti GM. Quantitative estimates of the optimal balance between digestible lysine and the true protein contents of broiler feeds. British Poultry Science 2016; 57: 538-550.

Baker DH. Advances in protein-amino acid nutrition of poultry. Amino Acids 2009; 37: 29-41.

Blethen DB, Wohlt JE, Jasaitis DK, et al. Feed Protein Fractions: Relationship to Nitrogen Solubility and Degradability. Journal of Dairy Science 1990; 73: 1544-1551.

Calsamiglia S, Stern MD. A three-step in vitro procedure for estimating intestinal digestion of protein in ruminants. Journal of Animal Science 1995; 73: 1459-1465.

Clunies M, Leeson S. in vitro Estimation of Dry Matter and Crude Protein Digestibility. Poultry Science 1984; 63: 89-96.

Fairbairn R, Alli I, Baker BE. Proteolysis Associated with the Ensiling of Chopped Alfalfa. Journal of Dairy Science 1988; 71: 152-158.

Herrera-Saldana R, Gomez-Alarcon R, Torabi M, et al. Influence of Synchronizing Protein and Starch Degradation in the Rumen on Nutrient Utilization and Microbial Protein Synthesis. Journal of Dairy Science 1990; 73: 142-148.

Herrera-Saldana R, Huber JT. Influence of Varying Protein and Starch Degradabilities on Performance of Lactating Cows. Journal of Dairy Science 1989; 72: 1477-1483.

Hou Y, Yin Y, Wu G. Dietary essentiality of “nutritionally non-essential amino acids” for animals and humans. Experimental Biology and Medicine 2015; 240: 997.

Hurrell RF, Carpenter KJ. Mechanisms of heat damage in proteins. British Journal of Nutrition 1974; 32: 589-604.

Ipharraguerre IR, Clark JH, Freeman DE. Varying protein and starch in the diet of dairy cows. I. Effects on ruminal fermentation and intestinal supply of nutrients. Journal of Dairy Science 2005; 88: 2537-2555.

Johnston J, Coon CN. A Comparison of Six Protein Quality Assays Using Commercially Available Protein Meals. Poultry Science 1979; 58: 919-927.

Johnston J, Coon CN. The Use of Varying Levels of Pepsin for Pepsin Digestion Studies with Animal Proteins. Poultry Science 1979; 58: 1271-1273.

Kong C, Adeola O. Evaluation of amino acid and energy utilization in feedstuff for swine and poultry diets. Asian-Australasian Journal of Animal Sciences 2014; 27: 917-925.

Kovalenko I V., Rippke GR, Hurburgh CR. Determination of amino acid composition of soybeans (Glycine max) by near-infrared spectroscopy. Journal of Agricultural and Food Chemistry 2006; 54: 3485-3491.

Li MM, Titgemeyer EC, Hanigan MD. A revised representation of urea and ammonia nitrogen recycling and use in the Molly cow model. Journal of Dairy Science 2019; 102: 5109-5129.

Mariotti F, Tomé D, Patureau Mirand P, et al. Converting Nitrogen into Protein-Beyond 6.25 and Jones’ Factors. Critical Reviews in Food Science and Nutrition; 48: 177-184.

National Academies of Sciences, Engineering, and Medicine; Division on Earth and Life Studies; Board on Agriculture and Natural Resources; Committee on Nutrient Requirements of Dairy Cattle. Nutrient Requirements of Dairy Cattle: Eighth Revised Edition. National Academies Press, 2021.

National Research Council, & Subcommittee on Poultry Nutrition. Nutrient Requirements of Poultry Ninth Revised Edition. National Academies Press ,1994.

National Research Council, Committee on Animal Nutrition, & Subcommittee on Dairy Cattle Nutrition. Nutrient Requirements of Dairy Cattle: 7th Revised Edition. National Academies Press, 2001.

Ng LT, Pascaud M. Plasma and tissue-free amino acids in the developing chick. Annals of Nutrition and Metabolism 1990; 34: 198-207.

Nocek JE, English JE. in situ Degradation Kinetics: Evaluation of Rate Determination Procedure. Journal of Dairy Science 1986; 69: 77-87.

Orskov ER, Mcdonald I. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. The Journal of Agricultural Science 1979; 92: 499-503.

Ravindran V, Bryden WL. Amino acid availability in poultry - in vitro and in vivo measurements. Australian Journal of Agricultural Research 1999; 50: 889-908.

Sniffen CJ, O’Connor JD, Van Soest PJ, et al. A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. Journal of Animal Science 1992; 70: 3562-3577.

Sriperm N, Pesti GM, Tillman PB. Evaluation of the fixed nitrogen-to-protein (N:P) conversion factor (6.25) versus ingredient specific N:P conversion factors in feedstuffs. Journal of the Science of Food and Agriculture 2011; 91: 1182-1186.

Stein HH, Sève B, Fuller MF, et al. Invited review: Amino acid bioavailability and digestibility in pig feed ingredients: Terminology and application. Journal of Animal Science 2007; 85: 172-180.

Stern MD, Bach A, Calsamiglia S. Alternative Techniques for Measuring Nutrient Digestion in Ruminants. Journal of Animal Science 1997; 75: 2256-2276.

van Soest PJ. Nutritional Ecology of the Ruminant. 2nd ed. Cornell University Press, 1994.

Vanhatalo A, Aronen I, Varvikko T. Intestinal nitrogen digestibility of heat-moisture treated rapeseed meals as assessed by the mobile-bag method in cows. Animal Feed Science and Technology 1995; 55: 139-152.

Vanzant ES, Cochran RC, Titgemeyer EC, et al. in vivo and in situ Measurements of Forage Protein Degradation in Beef Cattle. Journal of Animal Science 1996; 74: 2773-2784.

Vanzant ES, Cochran RC, Titgemeyer EC. Standardization of in situ Techniques for Ruminant Feedstuff Evaluation. Journal of Animal Science 1998; 76: 2717-2729.

White RR, Roman-Garcia Y, Firkins JL. Meta-analysis of postruminal microbial nitrogen flows in dairy cattle. II. Approaches to and implications of more mechanistic prediction. Journal of Dairy Science 2016; 99: 7932-7944.

Wilkerson VA, Klopfenstein TJ, Stroup WW. A collaborative study of in situ forage protein degradation. Journal of Animal Science 1995; 73: 583-588.

Xu S, Harrison JH, Riley RE. Characteristics of Nitrogen Fractions and Amino Acids of Feedstuffs Common to the Pacific Northwest. The Professional Animal Scientist 1996; 12: 223-237.

Yalçın S. Proteinler ve Metabolizması. In: Ergün A (ed) Hayvan Besleme ve Beslenme Hastalıkları. Ankara, 2020, pp. 61-103.

Zanton GI. Protein and amino acid nutrition. In: Large Dairy Herd Management. American Dairy Science Association, 2017, pp. 625-638.

Sayfalar

171-182

Yayınlanan

12 Ocak 2026

Lisans

Lisans