Sindirilebilirliğin Belirlenmesi ve Sindirilebilirliği Etkileyen Faktörler
Özet
Bu kitap bölümü, yemlerin sindirilebilirliğinin belirlenmesi ve sindirilebilirliği etkileyen faktörleri kapsamlı şekilde ele almaktadır. Sindirilebilirlik, yemin hayvan tarafından sindirilme, emilme ve metabolize edilme potansiyelini ifade eder ve verim, karlılık ile hayvan sağlığı açısından kritiktir. İn vivo yöntemler arasında klasik dışkı toplama tekniği, indikatör yöntemi ve fark yöntemi yer alır; her biri farklı pratik koşullara ve doğruluk düzeylerine sahiptir. İn vitro teknikler, rumen sıvısına dayalı RUSITEC, Tilley-Terry iki aşamalı prosedürü, gaz üretim tekniği ve Ankom filtre kese yöntemi gibi yaklaşımlarla kontrollü koşullarda hızlı ve tekrarlanabilir tahminler sunar. İn situ mobil naylon kese yöntemi ise rumende gerçekçi fermentasyon koşullarında parçalanmayı ölçer. Yakın kızılötesi spektroskopi (NIR) hızlı tahmin olanağı sağlasa da güçlü kalibrasyon gerektirir. Sindirilebilirliği; hayvana bağlı (tür, yaş, fizyolojik durum, yem tüketimi, hastalıklar), yeme bağlı (lif, protein, yağ, KEK, antinutrisyonel faktörler, hayvansal protein kaynakları) ve işleme bağlı (kimyasal muamele, öğütme, peletleme, ısıl işlem) değişkenler belirler. Uygun yöntem seçimi ve yem formülasyonunun optimizasyonu, performans ve sağlık hedeflerine ulaşmada temel stratejidir.
Referanslar
Arik HD, Inanc ZS, Kahraman O, Damar S, Akkaya AB. Effects of quebracho tannin supplementation in early lactation dairy cow rations on milk yield parameters, rumen fermentation, digestibility and blood parameters. Journal of Animal and Feed Sciences, 2024; 33(3): 321-330.
Atchade GST, Houndonougbo FM, Chrysostome CAAM, et al. Digestibility of feeds in broiler chicken (Galus galus linnaeus, 1758) in Africa: a review. International Journal of Biological and Chemical Sciences. 2019; 13(2): 1127-1139. doi: 10.4314/ijbcs.v13i2.43
Camacho LF, Silva TE, Palma MNN, et al. Evaluation of buffer solutions and urea addition for estimating the in vitro digestibility of feeds. Journal of Animal. Science; 2019; 97(2): 922–931. doi:10.1093/jas/sky464.
Dida MF, Challi DG, Gangasahay KY. Effect of feeding different proportions of pigeon pea (Cajanus cajan) and neem (Azadirachta indica) leaves on feed intake, digestibility, body weight gain and carcass characteristics of goats. Veterinary and Animal Science. 2019; 8, 100079. doi: 10.1016/j.vas.2019.100079
Fonnesbeck PV, Christiansen JL, Harris LE. Factors affecting digestibility of nutrients by sheep. Journal of Animal Science. 1981; 52(2): 363-376. doi: 10.2527/jas1981.522363x
Gilani GS, Xiao CW, Cockell KA. Impact of antinutritional factors in food proteins on the digestibility of protein and the bioavailability of amino acids and on protein quality. British Journal of Nutrition. 2012; 108(S2): 315-332. doi: 10.1017/S0007114512002371
Gülşen N, Umucalılar HD. Buzağıların Beslenmesi ve Beslenme Hastalıkları. Konya, S.Ü. Basımevi, 2009; 1-106.
Gülşen N, Umucalilar HD, İnal F, Hayirli, A. Impacts of calcium addition and different oil types and levels on in vitro rumen fermentation and digestibility. Archives of Animal Nutrition, 2006; 60(6), 443-453. doi:10.1080/17450390600973634
Hall MB, Mertens DR. In vitro fermentation vessel type and method alter fiber digestibility estimates. Journal of Dairy Science. 2008; 91(1): 301–307. doi: 10.3168/jds.2006-689
Jones PL, De Silva SS, 1998. Comparison of internal and external markers in digestibility studies involving the Australian freshwater crayfish, Cherax destructor Clark (Decapoda, Parastacidae). Aquaculture Research. 1998; 29: 487-493. doi: 10.1046/j.1365-2109.1998.00231.x
Kahraman O, Gülşen N, Inal F, Alataş MS, Inanc ZS, Ahmed I, Şişman D, Küçük, AE. (2023). Comparative analysis of in vitro fermentation parameters in Total mixed rations of dairy cows with varied levels of defatted black soldier Fly larvae (Hermetia illucens) as a substitute for soybean meal. Fermentation. 2023; 9(7): 652. doi:10.3390/fermentation9070652
Kahraman O, Gurbuz E, Inal F, Arık HD, Alatas MS, Inanc ZS, Ahmed I. (2023). Effects of Bacillus subtilis C-3102 addition on nutrient digestibility, faecal characteristics, blood chemistry and faecal Lactobacilli spp., Enterococci spp., and Escherichia coli in healthy dogs. Italian Journal of Animal Science. 2023; 22(1): 568-577. doi: 10.1080/1828051X.2023.2218871
Kavanagh S, Lynch PB, O’Mara F, et al. A comparison of total collection and marker technique for the measurement of apparent digestibility of diets for growing pigs. Animal Feed Science and Technology. 2001; 89(1-2): 49-58. doi: 10.1016/S0377-8401(00)00237-6
Khan MA, Mahr-Un-Nisa MUN, Sarwar M. Techniques measuring digestibility for the nutritional evaluation of feeds. International Journal of Agriculture and Biology. 2003; 5(1).91-94.
Lõbo Júnior MF, Rezende ASC, Saliba EOS, et al. Determination of apparent digestibility coefficients either by markers or total fecal collection techniques in dogs (in Portuguese). Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2001; 53(6): 691-694.
Martin EA, Nolan JV, Nitsan Z, et al 1998. Strategies to improve the nutritive value of rice bran in poultry diets. IV. Effects of addition of fish meal and a microbial phytase to duckling diets on bird performance and amino acid digestibility. British Poultry Science. 1998; 39(5): 612-621. doi: 10.1080/00071669888476
McCarthy JF, Aherne FX, Okai DB. Use of HCl insoluble ash as an index material for determining apparent digestibility with pigs. Canadian Journal of Animal Science. 1974; 54(1); 107-109. doi:10.4141/cjas74-016
McDonald P, Greenhalgh JFD, Morgan C, et al. Animal nutrition. 8th ed. Pearson Higher Ed. 2022. p. 249-253
McNab JM. Factors affecting the digestibility of nutrients. Proceedings of the Nutrition Society. 1975; 34(1): 5-11. doi: 10.1079/PNS19750003
Menke KH, Raab L, Salewski A. et al. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor in vitro. The Journal of Agricultural Science. 1979; 93(1): 217–222. doi: 10.1017/S0021859600086305
Sein T, Todd JR. Investigations into the use of indicator methods of estimating the digestibilities of feeds by ruminant animals. The Journal of Agricultural Science. 1998; 110(2): 315-320. doi: 10.1017/S002185960008134X
Silva TE, Detmann E, Camacho LF, et al. Comparação de métodos in vitro para quantificação da digestibilidade da matéria seca e da fibra em detergente neutro de forragens e concentrados. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2017; 69(6): 1635–1644. doi: 10.1590/1678-4162-9096
Tassone S, Fortina R, Peiretti PG. In vitro techniques using the DaisyII incubator for the assessment of digestibility: A review. Animals. 2020; 10(5): 775. doi: 10.3390/ani10050775
Titrek H, Arık HD. Süt İneği Rasyonlarında Sindirilebilen ve Sindirilmeyen NDF’nin İn Vitro ve İn Situ Metotlarla Belirlenmesi. Van Veterinary Journal, 2025; 36(2): 80-84.
Umucalılar HD, Gülşen N. Çiftlik hayvanlarında beslenme hastalıkları. Konya: Selçuk Üniv. Basımevi. 2005.
Umucalilar HD, Coşkun B, Gülşen, N. In situ rumen degradation and in vitro gas production of some selected grains from Turkey. Journal of animal physiology and animal nutrition, 2002; 86(9‐10): 288-297. doi:10.1046/j.1439-0396.2002.00381.x
Vukmirović Đ, Čolović R, Rakita S, et al. Importance of feed structure (particle size) and feed form (mash vs. pellets) in pig nutrition–A review. Animal Feed Science and Technology. 2017; 233: 133-144. 10.1016/j.anifeedsci.2017.06.016
Zewdie AK. The Different Methods of Measuring Feed Digestibility: A Review. EC Nutrition; 2019; 14(1): 68-74.