Mikroskop Altında Sperm-Oosit Birleştirilmesi ve İnkübatöre Yolculuğu

Yazarlar

Yeşim Kumtepe Çolakoğlu
Caroline Pirkevi Çetinkaya

Özet

Referanslar

Veeck LL, Oehninger S, Acosta AA, Muasher SJ. Sperm micro injection in a clinical in vitro fertilization program. In: Abstracts of the scientific papers at the forty-fifth annual meeting of the American Fertility Society. San Francisco; 1989. pp13-16.

Ng S-C, Bongso A, Ratnam SS. Microinjection of human oocytes: a technique for severe oligoasthenoteratozoospermia.FertilityandSterility.1991;56(6):1117-1123. https://doi.org/10.1016/S0015-0282(16)54726-8.

Palermo G, Joris H, Devroey P, et al. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992; 340(8810):17–18.

Palermo G, Joris H, Derde MP, Camus M, Devroey P, Van Steirteghem A. Sperm characteristics and outcome of human assisted fertilization by subzonal insemination and intracytoplasmic sperm injection. Fertility and Sterility. 1993;59(4):826-835.

Kocur OM, Xie P, Cheung S, Ng L, De Jesus A, Rosenwaks Z, Palermo GD. The intricate "ART" of ICSI. Journal of Assisted Reproduction and Genetics. 2025 Feb;42(2):349-365. doi: 10.1007/s10815-024-03322-3.

Joris H, Nagy Z, Van de Velde H, et al. Intracytoplasmic sperm injection: laboratory set-up and injection procedure. Hum Reprod 1998; 13(suppl 1): 76–86.

Pickering SJ, Braude PR, Johnson MH, et al. Transient cooling to room temperature can cause irreversible disruption of the meiotic spindle in the human oocyte. Fertil Steril 1990; 54(1):102–108.

Palermo GD, Schlegel PN, Colombero LT, et al. Aggressive sperm immobilization prior to intracytoplasmic sperm injection with immature spermatozoa improves fertilization and pregnancy rates. Hum Reprod 1996; 11(5):1023–1029.

Fishel S, Lisi F, Rinaldi L, et al. Systematic examination of immobilizing spermatozoa before intracytoplasmic sperm injection in the human. Hum Reprod 1995; 10(3):497–500.

Yanagida K, Morozumi K, Katayose H, Hayashi S, Sato A, Kimura Y. Successful fertilization and pregnancy following ICSI and oocyte activation with calcium ionophore in a case of globozoospermia. J Assist Reprod Genet. 2006;23(1):47–50.

Furuhashi, K., Saeki, Y., Enatsu, N., Iwasaki, T., Ito, K., Mizusawa, Y., Matsumoto, Y., Kokeguchi, S., & Shiotani, M. (2019). Piezo‐assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years. Reproductive Medicine and Biology, 18(4), 357–361. https://doi.org/10.1002/rmb2.12290

Caddy M, Popkiss S, Weston G, Vollenhoven B, Rombauts L, Green M, Zander-Fox D. PIEZO-ICSI increases fertilization rates compared with conventional ICSI in patients with poor prognosis. J Assist Reprod Genet. 2023 Feb;40(2):389-398. doi: 10.1007/s10815-022-02701-y. Epub 2022 Dec 31.

Singh M. et al. (2025). Successful live birth after fully automated AI-supported robotic ICSI: A case report. Fertility and Sterility Reports.

Mendizabal-Ruiz, G., Chavez-Badiola, A., Hernández-Morales, E., Valencia-Murillo, R., Ocegueda-Hernández, V., Costa-Borges, N., Cohen, J. (2025). A digitally controlled, remotely operated ICSI system: case report of the first live birth. Reproductive BioMedicine Online, 50(5), 104943. https://doi.org/10.1016/j.rbmo.2025.104943

Borges E Jr, de Almeida Ferreira Braga DP, de Sousa Bonetti TC, Iaconelli A Jr, Franco JG Jr. Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa. Fertil Steril. 2009 Jul;92(1):131-6. doi: 10.1016/j.fertnstert.2008.04.046. Epub 2008 Aug 9. PMID: 18692786..

Yoon SY, Eum JH, Lee JE, et al. Recombinant human phospholipase C zeta 1 induces intracellular calcium oscillations and oocyte activation in mouse and human oocytes. Hum Reprod. 2008;23(12):290–297.

Rybouchkin A, Dozortsev D, De Sutter P, Dhont M. Fertilization and early embryology: electrical activation of human oocytes after intracytoplasmic sperm injection. Hum Reprod. 1996;11(11):2177–2181.

Referanslar

Veeck LL, Oehninger S, Acosta AA, Muasher SJ. Sperm micro injection in a clinical in vitro fertilization program. In: Abstracts of the scientific papers at the forty-fifth annual meeting of the American Fertility Society. San Francisco; 1989. pp13-16.

Ng S-C, Bongso A, Ratnam SS. Microinjection of human oocytes: a technique for severe oligoasthenoteratozoospermia.FertilityandSterility.1991;56(6):1117-1123. https://doi.org/10.1016/S0015-0282(16)54726-8.

Palermo G, Joris H, Devroey P, et al. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992; 340(8810):17–18.

Palermo G, Joris H, Derde MP, Camus M, Devroey P, Van Steirteghem A. Sperm characteristics and outcome of human assisted fertilization by subzonal insemination and intracytoplasmic sperm injection. Fertility and Sterility. 1993;59(4):826-835.

Kocur OM, Xie P, Cheung S, Ng L, De Jesus A, Rosenwaks Z, Palermo GD. The intricate "ART" of ICSI. Journal of Assisted Reproduction and Genetics. 2025 Feb;42(2):349-365. doi: 10.1007/s10815-024-03322-3.

Joris H, Nagy Z, Van de Velde H, et al. Intracytoplasmic sperm injection: laboratory set-up and injection procedure. Hum Reprod 1998; 13(suppl 1): 76–86.

Pickering SJ, Braude PR, Johnson MH, et al. Transient cooling to room temperature can cause irreversible disruption of the meiotic spindle in the human oocyte. Fertil Steril 1990; 54(1):102–108.

Palermo GD, Schlegel PN, Colombero LT, et al. Aggressive sperm immobilization prior to intracytoplasmic sperm injection with immature spermatozoa improves fertilization and pregnancy rates. Hum Reprod 1996; 11(5):1023–1029.

Fishel S, Lisi F, Rinaldi L, et al. Systematic examination of immobilizing spermatozoa before intracytoplasmic sperm injection in the human. Hum Reprod 1995; 10(3):497–500.

Yanagida K, Morozumi K, Katayose H, Hayashi S, Sato A, Kimura Y. Successful fertilization and pregnancy following ICSI and oocyte activation with calcium ionophore in a case of globozoospermia. J Assist Reprod Genet. 2006;23(1):47–50.

Furuhashi, K., Saeki, Y., Enatsu, N., Iwasaki, T., Ito, K., Mizusawa, Y., Matsumoto, Y., Kokeguchi, S., & Shiotani, M. (2019). Piezo‐assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years. Reproductive Medicine and Biology, 18(4), 357–361. https://doi.org/10.1002/rmb2.12290

Caddy M, Popkiss S, Weston G, Vollenhoven B, Rombauts L, Green M, Zander-Fox D. PIEZO-ICSI increases fertilization rates compared with conventional ICSI in patients with poor prognosis. J Assist Reprod Genet. 2023 Feb;40(2):389-398. doi: 10.1007/s10815-022-02701-y. Epub 2022 Dec 31.

Singh M. et al. (2025). Successful live birth after fully automated AI-supported robotic ICSI: A case report. Fertility and Sterility Reports.

Mendizabal-Ruiz, G., Chavez-Badiola, A., Hernández-Morales, E., Valencia-Murillo, R., Ocegueda-Hernández, V., Costa-Borges, N., Cohen, J. (2025). A digitally controlled, remotely operated ICSI system: case report of the first live birth. Reproductive BioMedicine Online, 50(5), 104943. https://doi.org/10.1016/j.rbmo.2025.104943

Borges E Jr, de Almeida Ferreira Braga DP, de Sousa Bonetti TC, Iaconelli A Jr, Franco JG Jr. Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa. Fertil Steril. 2009 Jul;92(1):131-6. doi: 10.1016/j.fertnstert.2008.04.046. Epub 2008 Aug 9. PMID: 18692786..

Yoon SY, Eum JH, Lee JE, et al. Recombinant human phospholipase C zeta 1 induces intracellular calcium oscillations and oocyte activation in mouse and human oocytes. Hum Reprod. 2008;23(12):290–297.

Rybouchkin A, Dozortsev D, De Sutter P, Dhont M. Fertilization and early embryology: electrical activation of human oocytes after intracytoplasmic sperm injection. Hum Reprod. 1996;11(11):2177–2181.

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9 Haziran 2026

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